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• 2D/LT
Correction of Excitation Intensity
2D Spectrum (EEM)
Calculate Excitation Spectra
Low Temp. QY

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• For the correction of the wavelength dependent excitation intensity (lamp spectrum) of fluorescence spectrometers for a series of fluorescence spectra with known excitation wavelength.
  
  
• Load the lamp correction curve with the button [Load Lamp Spectrum]. Select the range of spectra to be corrected with the two drop down lists. The corrected spectra will replace the original spectra, their legend titles will be appended with "Excitation intensity corrected".
  
  
• Hint: The wavelength values can be entered manually for each spectrum in the menu item [General].
  
  

• Two-dimensional display of a series of fluorescence or excitation spectra. EEM means: Excitation Emission Matrix. The spectra should be connected to a wavelength and be corrected for excitation intensity before (see above). Example:2d_beispiel.zip (315KB)
  
  
• The mouse position is shown in the lower left corner as excitation, emission and intensity values. On the right there are some options for display. Excitation is oriented horizontally, emission goes vertically.
  You cannot print or save the graph at the moment, there are no axes.
  
  

• For calculating a series of excitation spectra from a series of fluorescence spectra. The spectra have to be connected to a wavelength and should be corrected for excitation intensity before(see above).
  
  
• Enter the range and stepwidth of detection wavelengths for the excitation spectra. Fluorescence spectra will be deleted if [Delete Fluorescence spectra] is checked.
  
  

• A useful explanation would go beyond the scope of this manual...